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KMID : 0861020190340040027
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Korea Journal of Herbology
2019 Volume.34 No. 4 p.27 ~ p.35
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Protective Effect of Dioscoreae Rhizoma Extracts in MIA-induced Rat.
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Kim Min-Ju
Park Hae-Jin
Kim Kyeong-Jo
Lee Jin-A
Shin Mi-Rae
Roh Seong-Soo
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Abstract
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Objectives : Osteoarthritis is characterized by degeneration of articular cartilage, which is characterized by chronic pain, stiffness and decrease range of motion. The present study was designed to compare the therapeutic effect of Dioscoreae Rhizoma water extract (DRW) and Dioscoreae Rhizoma 30% ethanol extract (DRE) on the monosodium iodoacetate (MIA)-induced osteoarthritis rats.
Methods : Osteoarthritis was induced by injection of MIA (50 ㎕ with 80 ㎎/㎖) into the knee joint cavity of rats. After adaptation period for seven days, rats were divided by 5 groups (n=10/group): normal group, control group, positive control (indomethacin 5 ㎎/㎏), DRW 200 ㎎/㎏ treated group, DRE 200 ㎎/㎏ treated group (n=10/group). The hind paw weight distribution was measured with the changes of reactive oxygen species (ROS), peroxynitrite (ONOO-) in articulation tissue. Also, the cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), tumor necrosis factor- alpha (TNFα), interleukin-6 (IL-6), matrix metalloproteinase-1 (MMP-1), and tissue inhibitor of metalloproteinases-1 (TIMP-1) were investigated by western blot analysis.
Results : The administration of DRW and DRE significantly decreased the hind paw weight distribution. The ROS and ONOO- levels of cartilaginous tissue were significantly decreased in DRW and DRE compared to control group. The results showed that DRE decreased inflammatory cytokines such as iNOS and TNFα. Also DRE decreased MMP-1 and increased TIMP-1.
Conclusions : Based on the above results, Dioscoreae Rhizoma extract seems to have the therapeutic effect on osteoarthritis via suppression of inflammation.
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KEYWORD
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Osteoarthritis, monosodium iodoacetate (MIA), Dioscoreae Rhizoma, Inflammation, MMP, TIMP
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